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an oligonucleotide (a linker) with the desired sequence and inserting the linker at the site of an endonuclease cut ( g. 1d). Far more impressive, however, is the ability to change a single speci c codon in order to replace any amino acid in the protein with any other amino acid. The process involves directed mutagenesis using arti cially created oligonucleotides. Basically, a short sequence of DNA (an oligonucleotide) is synthesized complementary to a region of the cloned gene, but with a change in one or more bases of a codon to specify a different amino acid. That oligonucleotide is then hybridized with the single-stranded form of the clone ( g. 2). Although one or more

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+ VDS _

Figure 1 A cloned gene can be mutated in several ways. (a) If a restriction endonuclease has two sites in the gene, the

VGG +_

Source + + + + + + + + + + + + Drain n n+ n+ Channel p Bulk (substrate)

intermediate piece can be spliced out. (b) If the endonuclease has only one site, the gene can be opened at that site, continued and limited digestion by exonucleases will delete part of the gene.

Fig 45: On the left are shown some common types of solid grinding wheels made using conventional abrasives

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The McGraw Hill Companies, 2001

When a gate voltage is applied, a conducting n-type channel is formed near the surface of the substrate; now current can flow from drain to source

+ VDS _

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(c) If an endonuclease has an offset region between its splice points of three or six nucleotides (one or two codons), that length can be inserted by repairing the single-stranded ends after cutting by the endonuclease. The resulting blunt ends can be spliced together. (Note that actually an ATT region has been converted to an ATTATT region. If reading codons along the DNA, the actual insertion is of a TAT codon.) (d) A linker of any length (usually the length of a speci c number of codons) can be inserted at a restriction site.

+ VDS _

[7], whereas on the right are shown typical shapes of standard metallic rim configurations for superabrasive wheels [17]

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The McGraw Hill Companies, 2001

G + + VGS S _

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bases will not match, hybridization can usually be facilitated by adjusting the pH or ionic strength of the solution. The hybridized oligonucleotide is then used as a primer for DNA replication; the whole plasmid is replicated, resulting in hybrid DNA. In subsequent DNA replications of the hybrid, both the original gene and the mutated DNA will be produced. The latter can be isolated by appropriate selection methods; it is a plasmid with a cloned gene that has the exact mutation the researcher wanted. Using techniques of this type, geneticists have made many advancements in understanding exactly how various components of an enzyme contribute to its function.

Source + + + + + + + + + + + + Drain n n+ n+ Pinched-off p channel Bulk (substrate)

G + VGS _

Figure 2 Site-directed mutagenesis can involve any nucleotide(s). In this case,

This coding system simplifies all technical specifications of the bonded abrasive required for the wheel, and it is normally designed slightly differently for conventional and superabrasive wheels as indicated in Figure 46 and Figure 47 In the superabrasive wheel coding system, the diamond/ CBN concentrations replace the structure and the thickness of the abrasive layer added at the end Usually, wheel coding systems use acronyms A, C, B and D to represent abrasive types for aluminium Table 42

If the drain-source voltage is kept fixed and the gate supply voltage is varied, the MOSFET will behave as a voltage-controlled resistor until the pinch-off condition is reached (see Figure 931(a))

If the drain and gate supply voltages are both varied, a family of curves (shown in Figure 931(b)) can be generated, illustrating the MOSFET cutoff, ohmic, saturation, and breakdown regions

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